Most of the natural products isolated at present are heterocyclic compounds, so heterocyclic compounds occupy an important position in the research of organic chemistry. A compound: 27828-71-3, is researched, SMILESS is O=C(O)C1=CN=CC(O)=C1, Molecular C6H5NO3Journal, Article, Research Support, Non-U.S. Gov’t, Cell Chemical Biology called A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast, Author is Li, Yi; Tian, Caiping; Liu, Keke; Zhou, Ying; Yang, Jing; Zou, Peng, the main research direction is Saccharomyces proteomics APEX2 alkyne phenol; APEX2; RNA; bioorthogonal reaction; mass spectrometry; mitochondria; proteomics; proximity labeling; spatial transcriptomics; yeast.COA of Formula: C6H5NO3.
The engineered ascorbate peroxidase (APEX) is a powerful tool for the proximity-dependent labeling of proteins and RNAs in live cells. Although widely use in mammalian cells, APEX applications in microorganisms have been hampered by the poor labeling efficiency of its biotin-phenol (BP) substrate. In this study, we sought to address this challenge by designing and screening a panel of alkyne-functionalized substrates. Our best probe, Alk-Ph, substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than BP. Through a combination of protein-centric and peptide-centric chemoproteomic experiments, we have identified 165 proteins with a specificity of 94% in the yeast mitochondrial matrix. In addition, we have demonstrated that Alk-Ph is useful for proximity-dependent RNA labeling in yeast, thus expanding the scope of APEX-seq. We envision that this improved APEX-labeling strategy would set the stage for the large-scale mapping of spatial proteome and transcriptome in yeast.
In addition to the literature in the link below, there is a lot of literature about this compound(5-Hydroxynicotinic acid)COA of Formula: C6H5NO3, illustrating the importance and wide applicability of this compound(27828-71-3).
Reference:
Chapter 1 An introduction to palladium catalysis,
Palladium/carbon catalyst regeneration and mechanical application method