Computed Properties of C6H5NO3. The mechanism of aromatic electrophilic substitution of aromatic heterocycles is consistent with that of benzene. Compound: 5-Hydroxynicotinic acid, is researched, Molecular C6H5NO3, CAS is 27828-71-3, about Probing the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase by using 8-substituted-FAD analogs. Author is Sucharitakul, Jeerus; Chaiyen, Pimchai; Ballou, David P.; Massey, Vincent.
The reaction mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) was investigated by using FAD-analogs substituted at the 8-position of the isoalloxazine ring. Replacing FAD of MHPCO with different FAD analogs having substituents at the 8-position did not greatly change the enzymic properties when compared with the native enzyme. Binding of the apoenzyme of MHPCO to the FAD analogs resulted in perturbation of the flavin absorbance and the fluorescence. The redox potential values of FAD analogs were shifted to more pos. values when bound to MHPCO. Using stopped-flow spectrophotometry, the oxidized enzyme was mixed with various concentrations of NADH, and the reaction was monitored at different wavelengths. The results indicate that the reaction of MHPCO reconstituted with FAD analogs proceeds through hydroxylation mechanisms similar to those for native enzyme.
Different reactions of this compound(5-Hydroxynicotinic acid)Computed Properties of C6H5NO3 require different conditions, so the reaction conditions are very important.
Reference:
Chapter 1 An introduction to palladium catalysis,
Palladium/carbon catalyst regeneration and mechanical application method